A-875

A-875是人类恶性黑色素瘤细胞系，最初是分离自一名40岁的人类女性黑色素瘤患者^[1]，对神经生长因子受体呈阳性反应，目前可用于建立动物研究模型。

科研用途[编辑]

1983年，有科研人员已经从A875细胞细胞膜的辛基葡萄糖苷（英语：Octyl glucoside）提取物中，纯化了NGF受体。最终A875细胞膜的可溶性提取物和纯化的NGF受体所进行的亲和标记（英语：Affinity label）实验表明，存在于NGF受体的两种主要的肽都可以与125I-NGF特异性交联 (specifically cross-linked) ^[2]。1990年，有研究人员比较A875细胞和大鼠嗜铬细胞瘤（英语：Pheochromocytoma）细胞系PC12细胞中NGF受体的特性。最终发现NGF的结合增强了悬浊液中A875细胞和PC12细胞的NGF受体横向扩散，而NGF对任何细胞系的NGF受体分布均没有任何影响^[3]。2017年，有学者利用A875细胞进行测试，发现SOX4（英语：SOX4）通过激活NF-κB/p65信号通路，促进黑色素瘤细胞的迁移和侵袭，并且指出SOX4可能是治疗黑色素瘤的潜在靶标基因^[4]。

参考资料[编辑]

^ Giard, DJ; Aaronson, SA; Todaro, GJ; Arnstein, P; Kersey, JH; Dosik, H; Parks, WP. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors.. Journal of the National Cancer Institute. 1973-11, 51 (5): 1417–23 [2019-12-30]. PMID 4357758. doi:10.1093/jnci/51.5.1417. （原始内容存档于2019-12-27）.
^ Puma, P; Buxser, SE; Watson, L; Kelleher, DJ; Johnson, GL. Purification of the receptor for nerve growth factor from A875 melanoma cells by affinity chromatography.. The Journal of biological chemistry. 1983-03-10, 258 (5): 3370–5 [2019-12-30]. PMID 6298234. （原始内容存档于2019-12-30）.
^ Venkatakrishnan, G; McKinnon, CA; Ross, AH; Wolf, DE. Lateral diffusion of nerve growth factor receptor: modulation by ligand-binding and cell-associated factors.. Cell regulation. 1990-07, 1 (8): 605–14 [2019-12-30]. PMID 1964090. doi:10.1091/mbc.1.8.605. （原始内容存档于2019-12-30）.
^ Cheng, Qiong; Wu, Jinfeng; Zhang, Yaohua; Liu, Xiao; Xu, Nan; Zuo, Fuguo; Xu, Jinhua. SOX4 promotes melanoma cell migration and invasion though the activation of the NF-κB signaling pathway. International Journal of Molecular Medicine. 2017-08, 40 (2): 447–453. doi:10.3892/ijmm.2017.3030.

外部链接[编辑]

Cellosaurus entry for A-875（页面存档备份，存于互联网档案馆）

[1] Giard, DJ; Aaronson, SA; Todaro, GJ; Arnstein, P; Kersey, JH; Dosik, H; Parks, WP. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors.. Journal of the National Cancer Institute. 1973-11, 51 (5): 1417–23 [2019-12-30]. PMID 4357758. doi:10.1093/jnci/51.5.1417. （原始内容存档于2019-12-27）.

[2] Puma, P; Buxser, SE; Watson, L; Kelleher, DJ; Johnson, GL. Purification of the receptor for nerve growth factor from A875 melanoma cells by affinity chromatography.. The Journal of biological chemistry. 1983-03-10, 258 (5): 3370–5 [2019-12-30]. PMID 6298234. （原始内容存档于2019-12-30）.

[3] Venkatakrishnan, G; McKinnon, CA; Ross, AH; Wolf, DE. Lateral diffusion of nerve growth factor receptor: modulation by ligand-binding and cell-associated factors.. Cell regulation. 1990-07, 1 (8): 605–14 [2019-12-30]. PMID 1964090. doi:10.1091/mbc.1.8.605. （原始内容存档于2019-12-30）.

[4] Cheng, Qiong; Wu, Jinfeng; Zhang, Yaohua; Liu, Xiao; Xu, Nan; Zuo, Fuguo; Xu, Jinhua. SOX4 promotes melanoma cell migration and invasion though the activation of the NF-κB signaling pathway. International Journal of Molecular Medicine. 2017-08, 40 (2): 447–453. doi:10.3892/ijmm.2017.3030.

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