A-875

A-875是人類惡性黑色素瘤細胞系，最初是分離自一名40歲的人類女性黑色素瘤患者^[1]，對神經生長因子受體呈陽性反應，目前可用於建立動物研究模型。

科研用途[编辑]

1983年，有科研人員已經從A875細胞細胞膜的辛基葡萄糖苷（英语：Octyl glucoside）提取物中，純化了NGF受體。最終A875細胞膜的可溶性提取物和純化的NGF受體所進行的親和標記（英语：Affinity label）實驗表明，存在於NGF受體的兩種主要的肽都可以與125I-NGF特異性交聯 (specifically cross-linked) ^[2]。1990年，有研究人員比較A875細胞和大鼠嗜鉻細胞瘤（英语：Pheochromocytoma）細胞系PC12细胞中NGF受體的特性。最終發現NGF的結合增強了懸濁液中A875細胞和PC12細胞的NGF受體橫向擴散，而NGF對任何細胞系的NGF受體分佈均沒有任何影響^[3]。2017年，有學者利用A875細胞進行測試，發現SOX4（英语：SOX4）通過激活NF-κB/p65信號通路，促進黑色素瘤細胞的遷移和侵襲，並且指出SOX4可能是治療黑色素瘤的潛在靶標基因^[4]。

參考資料[编辑]

^ Giard, DJ; Aaronson, SA; Todaro, GJ; Arnstein, P; Kersey, JH; Dosik, H; Parks, WP. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors.. Journal of the National Cancer Institute. 1973-11, 51 (5): 1417–23 [2019-12-30]. PMID 4357758. doi:10.1093/jnci/51.5.1417. （原始内容存档于2019-12-27）.
^ Puma, P; Buxser, SE; Watson, L; Kelleher, DJ; Johnson, GL. Purification of the receptor for nerve growth factor from A875 melanoma cells by affinity chromatography.. The Journal of biological chemistry. 1983-03-10, 258 (5): 3370–5 [2019-12-30]. PMID 6298234. （原始内容存档于2019-12-30）.
^ Venkatakrishnan, G; McKinnon, CA; Ross, AH; Wolf, DE. Lateral diffusion of nerve growth factor receptor: modulation by ligand-binding and cell-associated factors.. Cell regulation. 1990-07, 1 (8): 605–14 [2019-12-30]. PMID 1964090. doi:10.1091/mbc.1.8.605. （原始内容存档于2019-12-30）.
^ Cheng, Qiong; Wu, Jinfeng; Zhang, Yaohua; Liu, Xiao; Xu, Nan; Zuo, Fuguo; Xu, Jinhua. SOX4 promotes melanoma cell migration and invasion though the activation of the NF-κB signaling pathway. International Journal of Molecular Medicine. 2017-08, 40 (2): 447–453. doi:10.3892/ijmm.2017.3030.

外部連結[编辑]

Cellosaurus entry for A-875（页面存档备份，存于互联网档案馆）

[1] Giard, DJ; Aaronson, SA; Todaro, GJ; Arnstein, P; Kersey, JH; Dosik, H; Parks, WP. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors.. Journal of the National Cancer Institute. 1973-11, 51 (5): 1417–23 [2019-12-30]. PMID 4357758. doi:10.1093/jnci/51.5.1417. （原始内容存档于2019-12-27）.

[2] Puma, P; Buxser, SE; Watson, L; Kelleher, DJ; Johnson, GL. Purification of the receptor for nerve growth factor from A875 melanoma cells by affinity chromatography.. The Journal of biological chemistry. 1983-03-10, 258 (5): 3370–5 [2019-12-30]. PMID 6298234. （原始内容存档于2019-12-30）.

[3] Venkatakrishnan, G; McKinnon, CA; Ross, AH; Wolf, DE. Lateral diffusion of nerve growth factor receptor: modulation by ligand-binding and cell-associated factors.. Cell regulation. 1990-07, 1 (8): 605–14 [2019-12-30]. PMID 1964090. doi:10.1091/mbc.1.8.605. （原始内容存档于2019-12-30）.

[4] Cheng, Qiong; Wu, Jinfeng; Zhang, Yaohua; Liu, Xiao; Xu, Nan; Zuo, Fuguo; Xu, Jinhua. SOX4 promotes melanoma cell migration and invasion though the activation of the NF-κB signaling pathway. International Journal of Molecular Medicine. 2017-08, 40 (2): 447–453. doi:10.3892/ijmm.2017.3030.

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